If you lost root after updating your Transformer Prime with the latest OTA, we have good news: thanks to a new method found by Android hacker Saurik, you can now root once again.
So, you ready to make this happen? Let's get started.
First things first, you'll need to grab a couple of files.
Once you've downloaded the required files, plug your Prime into your computer, fire up adb, and enter the following commands (one at a time, of course):
adb push mempodroid /data/local
adb push su /data/local
adb shell chmod 777 /data/local/mempodroid
adb shell /data/local/mempodroid 0xd9ec 0xaf47 mount -o remount,rw '' /system
/data/local/mempodroid 0xd9ec 0xaf47 sh
At this point, you should be in a root shell session (indicated with the pound sign). Now, more commands!
cat /data/local/su > /system/xbin/su
chown 0.0 /system/xbin/su
chmod 06755 /system/xbin/su
adb shell rm /data/local/mempodroid
adb shell rm /data/local/su
And that's it! Head to the Market and install the Superuser app and you're good to go. Enjoy!